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Draft a comprehensive differential diagnosis for acute substernal chest pain in a 45-year-old male with hypertension and hyperlipidemia, diaphoresis, exertional onset, and normal initial ECG/troponin; include life-threatening causes first, key distinguishing features, and immediate diagnostic next steps.

Draft a detailed differential diagnosis for new-onset unilateral throbbing headache with visual aura and brief monocular vision loss in a 32-year-old female on oral contraceptives; include red-flag symptoms, stroke/TIA considerations, and recommended initial workup.

Draft an assessment and plan for stage-2 hypertension (BP 162/98) in an obese 52-year-old with suspected obstructive sleep apnea; include ASCVD risk estimate, lifestyle prescriptions, first-line pharmacotherapy with drug/dose/targets, baseline labs/monitoring, and 4–6 week follow-up.

Draft an assessment and plan for type 2 diabetes follow-up with A1c 9.2% on metformin 1,000 mg BID and eGFR 65; compare adding a GLP-1 receptor agonist vs an SGLT2 inhibitor, address weight loss goals, hypoglycemia education, vaccinations, and micro/macrovascular screening.

Draft a history and physical for a patient presenting with chest pain, including HPI using OLDCARTS, pertinent ROS, PMH/meds/allergies, FH/SH, focused cardiac/pulmonary exam, initial labs and imaging orders, prioritized differential, HEART score risk stratification, and early management.

Draft a history and physical for a new patient with progressive exertional dyspnea, orthopnea, and bilateral leg edema; include differential (heart failure, COPD, PE, anemia, renal disease), targeted exam maneuvers, and first-line diagnostics and management.

Draft a progress note for COPD follow-up two weeks after an acute exacerbation treated with prednisone and antibiotics; include symptom trajectory, CAT score (if available), inhaler technique/adherence, oxygen needs, spirometry plan, smoking cessation counseling, and return precautions.

Draft a progress note for post-operative day 2 after total knee arthroplasty; include pain control strategy, DVT prophylaxis, PT/OT milestones, wound status, bowel/urinary function, labs/vitals review, complications screening, and discharge readiness.

Draft a discharge summary for community-acquired pneumonia admission; include admission/discharge dates, presenting symptoms, radiology/labs, pathogen if identified, inpatient therapies and response, discharge medications with durations, pending tests, follow-up appointments, and return precautions.

Draft a discharge summary for myocardial infarction managed with PCI and drug-eluting stent placement; include cath findings, complications, inpatient course, discharge medications (DAPT, statin, beta-blocker, ACEi/ARB), cardiac rehab referral, and follow-up plan.

Draft discharge instructions for a patient hospitalized with acute decompensated heart failure; include daily weights, sodium and fluid restriction amounts, medication list with dosing, signs of worsening heart failure, follow-up appointments, and lab monitoring plan.

Draft discharge instructions for an asthma exacerbation; include controller and reliever regimen, steroid taper schedule, spacer technique, trigger avoidance, a written asthma action plan, return precautions, and follow-up timing.

Draft a patient handout on managing high blood pressure in plain language; explain what BP numbers mean, key lifestyle steps (DASH diet, sodium limits, alcohol moderation), home BP monitoring instructions, medication adherence tips, and when to call the doctor.

Draft a patient handout for type 2 diabetes education in plain language; cover A1c goals, the plate method, physical activity targets, self-monitoring of blood glucose, hypoglycemia signs and the 15-15 rule, foot care basics, and a vaccination checklist.

Draft a summary of the latest CDC and WHO guidelines for managing community-acquired pneumonia in adults, including empiric antibiotic choices, duration of therapy, and follow-up recommendations.

Draft a plain-language overview of the ADA and AACE guidelines for diabetes management, focusing on A1c goals, weight management, and the use of GLP-1 receptor agonists and SGLT2 inhibitors.

Draft a summary of the 2024 USPSTF breast cancer screening recommendations, including updated screening intervals, risk-based approaches, and the role of MRI for high-risk patients.

Draft a concise summary of NCCN guidelines for melanoma management, including staging, surgical margins, adjuvant therapy, and checkpoint inhibitor recommendations.

Draft treatment alternatives for community-acquired MRSA skin infections in patients allergic to sulfa drugs; include oral and IV options, dosing, and duration.

Draft treatment alternatives for patients with type 2 diabetes who are unable to tolerate GLP-1 receptor agonists due to nausea; include medication substitutions and lifestyle recommendations.

Draft a summary of options for treating refractory depression, comparing ketamine infusions, transcranial magnetic stimulation (TMS), and electroconvulsive therapy (ECT).

Draft treatment options for advanced prostate cancer, including hormonal therapy, radiopharmaceuticals, and novel androgen receptor pathway inhibitors.

Draft a summary of recent research on mRNA vaccine technology beyond COVID-19, including applications in oncology and autoimmune disease prevention.

Draft an easy-to-understand overview of long COVID research, summarizing major symptoms, hypothesized mechanisms, and emerging treatment approaches.

Draft a summary of current research on Alzheimer’s disease biomarkers, including amyloid and tau imaging, and discuss how they influence diagnosis and trial design.

Draft an explanation of the interaction between warfarin and antibiotics such as ciprofloxacin or TMP-SMX, including INR effects and management recommendations.

Draft a summary evaluating drug interactions for patients taking Paxlovid and common cardiovascular drugs; include mechanism and recommended dose adjustments.

Draft a summary of the common and serious side effects of semaglutide, including GI intolerance, gallbladder disease, and thyroid-related warnings.

Draft an overview of potential side effects associated with long-term corticosteroid use, including osteoporosis, glucose intolerance, and adrenal suppression.

Draft a summary of essential lab tests to order in suspected acute pancreatitis, including amylase, lipase, liver enzymes, and metabolic panels, with interpretation guidance.

Draft laboratory workup recommendations for suspected hypothyroidism, including TSH, free T4, thyroid antibodies, and when to consider secondary causes.

Draft an explanation of which labs should be monitored in patients receiving methotrexate therapy, including CBC, LFTs, renal function, and frequency of monitoring.

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Research Article: Bridging borders: the virtual biorepository system, a sustainable global biobanking system for epidemic threats preparedness

Date Published: 2025-10-28

Abstract:
Frequent outbreaks of diseases such as Ebola, dengue fever, Zika, and COVID-19 have highlighted the urgent need for equitable and timely access to high-quality biological specimens and associated data for diagnostics, vaccine development, and research. However infectious disease collections and biorepositories frequently have narrow mandates, prioritize internal goals, and lack global coordination. The Virtual Biorepository System (VBS) aims to transform this landscape by connecting local biorepositories, especially in low- and middle-income countries (LMICs), into a federated, trust-based network that promotes global equity, access, and resilience during public health emergencies. Under the CONTAGIO (Cohort Networks to be Activated during Outbreaks) program, a pilot known as “10x10” was launched to test the feasibility of the VBS model. Ten founding member institutions from LMICs were selected based on location, specimen sharing and biorepository experience, interest, and willingness to engage for at least three years. Each site agreed to collect and share ten mL of plasma or serum from ten individuals, allowing assessment of molecular/immunological testing capacity and shipping logistics, and development of governance and a sustainable business model. Coordination was managed virtually through regular meetings and workshops, with the Center for Global Health (CGH) being the managing partner as well as co-technical lead along with University Aix-Marseille. Participating sites included national reference labs, accredited biorepositories, academic institutions, and newer biorepositories. Motivations for participation included networking opportunities, capacity building, contributing to epidemic preparedness, and increasing knowledge of regulatory and logistical challenges related to specimen sharing. Benefits reported by sites emphasized enhanced collaboration, technical capacity development, and alignment with global outbreak response efforts. The 10x10 pilot demonstrates the practical implementation of the VBS model and identifies known and emerging barriers to specimen access. Its LMIC-focused design enhances the representativeness of samples for diagnostic and vaccine development, addressing long-standing equity gaps. Lessons from the pilot will inform scale-up and refinement of governance, data harmonization, and sustainability strategies. The VBS, through the 10x10 pilot, has shown strong early feasibility and collaboration, reinforcing the demand for an equitable biorepository system for preparedness. Continued development under the CONTAGIO program will build global biobanking capacity and improve epidemic preparedness, particularly in underrepresented regions.

Introduction:
Frequent outbreaks of diseases such as Ebola, dengue fever, Zika, and COVID-19 have highlighted the urgent need for equitable and timely access to high-quality biological specimens and associated data for diagnostics, vaccine development, and research. However infectious disease collections and biorepositories frequently have narrow mandates, prioritize internal goals, and lack global coordination. The Virtual Biorepository System (VBS) aims to transform this landscape by connecting local biorepositories,…

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